摘要:内切葡聚糖酶是一种常见的纤维素酶，本文通过液态发酵技术，针对实验室的一株重组内切葡聚糖酶产生菌株进行发酵分析，以发酵产物中纤维素酶活为指标，分析重组酶的活性特征。重组内切葡聚糖酶产生菌株液态发酵及诱导后，改变酶反应条件，采用羧甲基纤维素钠（CMC-Na）法测定其酶活，得出此菌株经诱导2h，在pH5.0左右，温度50℃的反应条件下酶活达到最高为44.67U/ml，同时Mn2+对该酶具有促进作用。本研究有助于从发酵产酶的角度更深入地对重组内切葡聚糖酶所具备的特性进行分析，更好地了解所构建的内切葡聚糖酶的特点，对于新型内切葡聚糖酶的开发有推动作用。

关键词 P. ananatis；纤维素酶；内切葡聚糖酶；活性特征

Abstract:Endoglucanase is a common cellulose. Taking fermentation product in cellulase activity as an index, enzyme activity of a recombinant endoglucanase strain was analyzed by liquid fermentation technology. Recombinant endoglucanase enzymes producing strain liquid fermentation and after induction, changes in enzyme reaction conditions, using sodium carboxymethyl cellulose ( CMC-Na ) method for the determination of the enzyme activity, the strain after induced by 2h, at around pH5.0, temperature 50℃ under the reaction conditions of enzyme activity reached the highest by 44.67U/ml, while Mn2+ on the enzyme has stimulative effect. This study provides more in-depth perspective on fermentation of recombinant endoglucanase and a better understanding of the endoglucanase characteristics for novel endoglucanase development.

Keywords   P. ananatis  Cellulase  Endoglucanase  Activity characteristics