摘要：目的：本课题旨在研究葡萄籽原花青素提取物对DDP所致大鼠生殖毒性的保护作用，从而为保护机体正常组织且不影响DDP抗肿瘤效果提供一种临床上更为有效的辅助用药。

　　　方法：成熟雄性SD大鼠50只，随机分为5组。分别为对照组、DDP处理组、DDP+GSPE(200mg/kg)组、DDP+GSPE(400mg/kg)组、GSPE(400mg/kg)组。灌服GSPE后第9天DDP组和DDP+GSPE组一次性腹腔注射DDP(7.0mg/kg)，对照组注射等量生理盐水。实验第11天晚上断食，第12天进行动物宰杀。取睾丸及附睾，迅速用液氮冷冻，于-80℃保存。观察各组大鼠组织切片、精子畸形率、谷胱甘肽过氧化物酶（GSH-PX）、超氧化物歧化酶（SOD）、丙二醛指标。

　　　结果：葡萄籽原花青素提取物可以控制精子畸变；改善DDP引起的睾丸组织SOD和GSH-PX活性下降,降低组织中丙二醛的含量。

　　　结论：GSPE经口给予能明显预防DDP所致的生殖毒性。其机制可能是GSPE使DDP在体内产生的自由基减少，脂质过氧化程度减轻，提高机体抗氧化能力。

关键词：葡萄籽原花青素提取物 DDP 生殖毒性

Abstract:Objective: This project is to study the protective effect of grape seed proanthocyanidin extract on cisplatin-induced reproductive toxicity, so as to protect the body from normal tissue without affecting the antitumor effect of cisplatin to provide a clinically more effective adjuvant. 

　　　Methods: Mature male SD rats were 50, were randomly divided into 5 groups. For the control group, DDP treatment group, DDP + GSPE (200g/kg) group，DDP + GSPE (400g/kg) group, GSPE (400g/kg) group. Gavage the GSPE After nine days of DDP group and the DDP + GSPE, it was a single intraperitoneal injection of the DDP (7.0mg/kg), control group was injected with normal saline. Experiment 11 days of fasting, 12 days for animals slaughtered. Testis and epididymis, and quickly frozen in liquid nitrogen and stored at -80 ℃. Observe the rats in each group tissue sections, sperm deformity rate, glutathione peroxidase (GSH-PX), superoxide dismutase (SOD), malondialdehyde indicators.

　　　Results: grape seed proanthocyanidin extract can control the sperm aberration; to reduce DDP-induced organ coefficient levels; improve the DDP-induced testicular tissue SOD and GSH-PX activity decreased, reducing the content of malondialdehyde

　　　Conclusion: GSPE given orally significantly to the prevention of DDP-induced reproductive toxicity. The mechanism may be to The GSPE make DDP to reduce the free radicals generated in vivo, reduce the degree of lipid peroxidation, increase the antioxidant capacity.

Keywords: grape seed proanthocyanidin  extract reproductive toxicity of cisplatin